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There exists an overlap in the ISGs triggered by type I and type II IFNs Type I IFNs activate the JAK/STAT signal transduction pathway by binding to the IFN-a/b receptor (IFNAR) The cytoplasmic tails of IFNAR are associated with tyrosine kinases (JAK and TYK), which phosphorylate signal transducers and activators of transcription (STAT)5 STATs are latent transcription factors that, upon phosphorylation, dimerize and form complexes with interferon response factors (IRFs) These complexes move to the nucleus and bind to interferon-stimulated regulatory elements (ISRE)6 Several genes are then transcriptionally stimulated Of these gene products, many contribute to the antiviral actions of the host immune response PKR is a double-stranded RNA (dsRNA)-dependent serine/threonine kinase In the presence of dsRNA, most likely produced from the viral genome itself or formed from its replication or convergent transcription, PKR phosphorylates the a subunit of the eukaryotic initiation factor eIF-2.

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When eIF-2a is phosphorylated, it is unable to recycle and the translation of proteins is arrested, thereby inhibiting viral reproduction Also, activation of PKR results in the phosphorylation of substrates necessary to initiate the transcription factor NF-kB Once freed from restraint, NF-kB can enter the nucleus of the cell and bind to the IFN-b promoter7Related: EAN-8 Generating NET , NET UPC-E Generating , Generate ISBN NET.

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