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circumstances, these proteins play a major role in transporting another kind of protein called antigens within a cell (and thus they have an even more colorful nickname, chaperones) Antigens, for their part, stimulate the body s immune system to respond to infection or disease In theory, you could extract and purify the heat-shock proteins that had chaperoned an antigen that stimulated a response to a certain cancer Then the extracted heat-shock proteins could be made into a vaccine that would contain some trace of that specific antigen and its cancer the antigenic fingerprint of that cancer If a patient got that vaccine, unique to his or her cancer, the immune system might be reprogrammed to home in on cancer cells bearing the antigenic fingerprint It would not prevent anyone from getting cancer, but it could stop the cancer from spreading That was the theory, anyway.

A number of universities and research institutes in the United States and Europe were also studying the heatshock protein process, and so far the buzz about Antigenics among scientists and on Wall Street was cautiously positive The vaccine, which was called Oncophage, had already proved itself in animal experiments, in tests for safety, and even in the first stage of clinical trials on cancer patients A trial of colorectal cancer patients had just reported some good news about survival rates Now 650 people with kidney cancer and 350 with skin cancer were participating in further tests at more than 130 sites around the world As soon as doctors removed a patient s tumor, the specimen was frozen in dry ice and rushed to Antigenics labs in Woburn and Lexington, Massachusetts, both near Boston.

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There, scientists had 24 hours in which to extract the heat-shock proteins they needed a minimum of seven grams of tumor and process them into a vaccine For the next three weeks the vaccines were tested for purity, sterility, and composition Finally, at least four vials were flown back to each patient and injected one a week for four weeks, then biweekly The stuff looked like a small glass of Sprite Of course, these were only tests Oncophage was still far from being a safe, workable drug, let alone a cure for cancer The Antigenics scientists figured they would need at least two more years of clinical testing, checking to see if the cancer had spread, before they would be ready to seek official FDA approval So there really wasn t much reason to be hanging out at the FDA s headquarters in Rockville.

June 10, 2004

But Antigenics had requested this special meeting because a problem had cropped up The FDA had recently reorganized Some 200 reviewers who specialized in protein-based drugs, including staffers who had been.

Be sure that your graphics program is adept at handling the three graphic formats used on the Web: GIFs, JPEGs, and PNG images. Macromedia makes Fireworks, a graphics editor designed for the Web that integrates nicely with Dreamweaver. In fact, it integrates so nicely, this book includes an entire chapter on it, 22.

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than linking the trunk and leaf pages together in one large, at linked list, SQLite only has to journal the trunk pages When data is deleted from a database, each whole page that becomes free (ie that contains only deleted data) is added to the free page list If the database has auto-vacuum set to none then the free pages remain on the free page list when the transaction completes When a subsequent database operation for example, an INSERT statement requires pages to be allocated, SQLite rst checks the free page list to see whether there are any free pages that can be reused (note that both the trunk and the leaf pages in the free page list are eligible for reuse) If there are free pages available then SQLite uses them and the free list page is updated to no longer include those pages.

Reusing free pages in a database is clearly preferable to allocating additional disk space for new pages That said, the creator of a database may prefer to con gure it so that when a page becomes free the space that it occupies can be reclaimed by the le system, which can result in the size of the database le being reduced If the database has auto-vacuum set to full then at the end of every transaction on the database all pages on the free page list are automatically moved to the end of the database le and the le is then truncated to remove the free pages.

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