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alcohols, and certain organic compounds such as urea and formamide. All of these are typically used in the laboratory. In cells, there are enzymes that can catalyze the unwinding of DNA. The most common of these is an enzyme called helicase. We will see later that other enzymes also affect the unwinding of DNA, but indirectly, through modifying the DNA s tertiary structure. In addition to this, some proteins that bind to DNA specifically stabilize the unwound state, and so contribute to a favorable free-energy change for unwinding the helix. DNA melting studies are usually done by slowly raising the temperature of a sample of DNA. Alternatively one can gradually add a chemical denaturing agent. The melting transition can be detected in a variety of ways. The two most common are measuring absorbance at a wavelength of 260 nm or measuring the average excess heat capacity. Let s talk about each of these. Nucleotide bases absorb ultraviolet light with an absorption maximum at 260 nm. When the bases are stacked in a helical structure (even single stranded), some of the absorbance is quenched. This is because the electrons that participate in basestacking interactions are energetically constrained from absorbing photons. As the helix melts, base-stacking interactions are lost and absorbance increases, reaching a maximum when all of the molecules in the sample are completely in the coil state. This can be seen in Fig. 10-8. The halfway point of the melting transition is called the melting temperature and is designated Tm. It is at this temperature where we interpret it to mean that the molecules are, on average, halfway melted. However, depending on

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A spinner bot was first used on The South Bay Mauler (Robot Wars, 1994). Hazard, Odin, Ziggo, Tortise, Turbo, and Blendo are spinner bots. These bots feature a heavy spinning bar or disk, possible with hammer heads, chisels, maces, or other protrusion pieces attached.

Instead, it was an enhancement to an already highly successful language This approach to language development beginning with an existing language and moving it forward established a trend that continues today..

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As the preceding examples have shown, methods inside a generic class can make use of a class type parameter and are, therefore, automatically generic relative to the type parameter. However, it is possible to declare a generic method that uses one or more type parameters of its own. Furthermore, it is possible to create a generic method that is enclosed within a non-generic class. Let s begin with an example. The following program declares a non-generic class called ArrayUtils and a static generic method within that class called CopyInsert( ). The CopyInsert( ) method copies the contents of one array to another, inserting a new element at a specified location in the process. It can be used with any type of array.

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To say that the electrical system of an EV is its most important part is not an oxymoron far from it. The idea is to leave as much of the internal combustion vehicle instrumentation wiring as you need intact, and to carefully add the high-voltage, high-current wiring required by your EV conversion. Do a great job on your EV conversion s high-voltage, high-current wiring and it will reward you with years of trouble-free service. Do it in a sloppy manner and, aside from the obvious boom or poof (accompanied by smoke), you open yourself to a world of strange malfunctions. The hidden beauty of any home-built EV conversion is that there are only two places to lay blame if anything has gone wrong: look in the mirror and under the hood. This section will cover the electrical system that interconnects the motor, controller, batteries, and charger along with its key high-voltage, high-current power, and lowvoltage, low-current instrumentation components. Figure 9-7 shows you the system at a glance. We ll look at the components that go into high-current and low-current side separately, then discuss wiring it all together.

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